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optical aggregometer aggram  (Helena Laboratories)

 
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    Helena Laboratories optical aggregometer aggram
    Optical Aggregometer Aggram, supplied by Helena Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optical aggregometer aggram/product/Helena Laboratories
    Average 90 stars, based on 1 article reviews
    optical aggregometer aggram - by Bioz Stars, 2026-05
    90/100 stars

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    Effect of BDMPs on platelets. (A) Human platelets incubated with PKH26-labeled BDMPs and a CD61 antibody (10 minutes at 37°C) were gated on CD61 positivity and analyzed for PKH26 fluorescence by flow cytometry (1-way ANOVA, n = 12, *P < .01 compared with baseline). (B) Scanning electron microscopic images of platelets immobilized onto fibrinogen and incubated with either (left) PBS (bar = 1 μm) or (right) BDMPs (bar = 1 μm). (C) Calcium influx in platelets incubated with 25 000/μL BDMPs for the indicated times (n = 6, repeated-measures ANOVA, *P < .001, the values are after background subtraction). (D) CD62p expression was measured on platelets incubated with 25 000/μL BDMPs for 30 minutes at 37°C in the presence and absence of type I collagen (n = 6, paired t test). (E) Annexin V binding to platelets (CD61+) treated with BDMPs was measured by flow cytometry (n = 6, 1-way ANOVA, *P < .001 compared with baseline). (F) Human platelets were monitored for aggregation in an optical <t>aggregometer</t> after 25 000/μL BDMPs were added to 100 μL PRP with and without collagen (n = 6, paired t test).
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    Effect of BDMPs on platelets. (A) Human platelets incubated with PKH26-labeled BDMPs and a CD61 antibody (10 minutes at 37°C) were gated on CD61 positivity and analyzed for PKH26 fluorescence by flow cytometry (1-way ANOVA, n = 12, *P < .01 compared with baseline). (B) Scanning electron microscopic images of platelets immobilized onto fibrinogen and incubated with either (left) PBS (bar = 1 μm) or (right) BDMPs (bar = 1 μm). (C) Calcium influx in platelets incubated with 25 000/μL BDMPs for the indicated times (n = 6, repeated-measures ANOVA, *P < .001, the values are after background subtraction). (D) CD62p expression was measured on platelets incubated with 25 000/μL BDMPs for 30 minutes at 37°C in the presence and absence of type I collagen (n = 6, paired t test). (E) Annexin V binding to platelets (CD61+) treated with BDMPs was measured by flow cytometry (n = 6, 1-way ANOVA, *P < .001 compared with baseline). (F) Human platelets were monitored for aggregation in an optical <t>aggregometer</t> after 25 000/μL BDMPs were added to 100 μL PRP with and without collagen (n = 6, paired t test).
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    Effect of BDMPs on platelets. (A) Human platelets incubated with PKH26-labeled BDMPs and a CD61 antibody (10 minutes at 37°C) were gated on CD61 positivity and analyzed for PKH26 fluorescence by flow cytometry (1-way ANOVA, n = 12, *P < .01 compared with baseline). (B) Scanning electron microscopic images of platelets immobilized onto fibrinogen and incubated with either (left) PBS (bar = 1 μm) or (right) BDMPs (bar = 1 μm). (C) Calcium influx in platelets incubated with 25 000/μL BDMPs for the indicated times (n = 6, repeated-measures ANOVA, *P < .001, the values are after background subtraction). (D) CD62p expression was measured on platelets incubated with 25 000/μL BDMPs for 30 minutes at 37°C in the presence and absence of type I collagen (n = 6, paired t test). (E) Annexin V binding to platelets (CD61+) treated with BDMPs was measured by flow cytometry (n = 6, 1-way ANOVA, *P < .001 compared with baseline). (F) Human platelets were monitored for aggregation in an optical aggregometer after 25 000/μL BDMPs were added to 100 μL PRP with and without collagen (n = 6, paired t test).

    Journal: Blood

    Article Title: Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury

    doi: 10.1182/blood-2014-09-598805

    Figure Lengend Snippet: Effect of BDMPs on platelets. (A) Human platelets incubated with PKH26-labeled BDMPs and a CD61 antibody (10 minutes at 37°C) were gated on CD61 positivity and analyzed for PKH26 fluorescence by flow cytometry (1-way ANOVA, n = 12, *P < .01 compared with baseline). (B) Scanning electron microscopic images of platelets immobilized onto fibrinogen and incubated with either (left) PBS (bar = 1 μm) or (right) BDMPs (bar = 1 μm). (C) Calcium influx in platelets incubated with 25 000/μL BDMPs for the indicated times (n = 6, repeated-measures ANOVA, *P < .001, the values are after background subtraction). (D) CD62p expression was measured on platelets incubated with 25 000/μL BDMPs for 30 minutes at 37°C in the presence and absence of type I collagen (n = 6, paired t test). (E) Annexin V binding to platelets (CD61+) treated with BDMPs was measured by flow cytometry (n = 6, 1-way ANOVA, *P < .001 compared with baseline). (F) Human platelets were monitored for aggregation in an optical aggregometer after 25 000/μL BDMPs were added to 100 μL PRP with and without collagen (n = 6, paired t test).

    Article Snippet: Platelet aggregation induced by 1 × 10 4 /μL of BDMPs, in the presence and absence of type I collagen (Helena Laboratories, Beaumont, TX), was monitored at 37°C for 10 minutes in an AggRAM optical aggregometer (Helena Laboratories).

    Techniques: Incubation, Labeling, Fluorescence, Flow Cytometry, Expressing, Binding Assay